Optimization

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Optimization of Botryococcus Growth and Hydrocarbon Production

Media Optimization

Optimization of Environmental Factors

Qin J.G., Lin Y., Optimization of the Growth Environment of Botryococcus braunii Strain CHN 357. Journal of Freshwater Ecology 21(1):169-176. doi:10.1080/02705060.2006.9664110

Xu, L. , Wang, F. , Guo, C. and Liu, C. (2012), Improved algal oil production from Botryococcus braunii by feeding nitrate and phosphate in an airlift bioreactor. Eng. Life Sci., 12: 171-177. doi:10.1002/elsc.201100110

Yoshimura T., Okada S., & Honda M. (2013). Culture of the hydrocarbon producing microalga Botryococcus braunii strain Showa: optimal CO2, salinity, temperature, and irradiance conditions. Bioresour Technol, 133:232-9. doi: 10.1016/j.biortech.2013.01.095.

Nitrogen Focus

Singh, Y. & Kumar, H.D. World (1992). Lipid and hydrocarbon production by Botryococcus spp. under nitrogen limitation and anaerobiosis. J Microbiol Biotechnol, 8: 121. doi:10.1007/BF01195829

Seed Culture

Harvesting

Lee K., & Kim T. (2002). Effects of harvesting method and growth stage on the flocculation of the green alga Botryococcus braunii. Letters in Applied Microbiology 27(1):14-18. doi:10.1046/j.1472-765X.1998.00375.x"