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Optimization of Botryococcus Growth and Hydrocarbon Production[edit]

Media Optimization[edit]

Al-Hothaly K, Mouradov A., Mansur A., May B., Ball A., & Adetutu E. (2015). The Effect of Media on Biomass and Oil Production in Botryococcus braunii Strains Kossou-4 and Overjuyo-3. International Journal of Clean Coal and Energy. 04. 11-22. 10.4236/ijcce.2015.41002.

Asma, J., Yusoff F., & Srikanth R.M. (2015). Growth rate assessment of high lipid producing microalga Botryococcus braunii in different culture media. Iranian Journal of Fisheries Sciences. 14: 436-445.

Tran, HL., Kwon, JS., Kim, ZH. et al. (2010). Statistical Optimization of Culture Media for Growth and Lipid Production of Boytryococcus braunii LB572. Biotechnol Bioproc E, 15: 277. https://doi.org/10.1007/s12257-009-0127-7

Velichkova, K., Sirakov I., & Georgiev G. (2013). Cultivation of Botryococcus braunii strain in relation of its use for biodiesel production. J. BioSci. Biotech., SE/ONLINE. 157-162.

Nitrogen in Media[edit]

Dayananda C, Sarada R., Shamala T.R., & Gokare R. (2006). Influence of Nitrogen Sources on Growth, Hydrocarbon and Fatty Acid Production by Botryococcus braunii. Asian Journal of Plant Sciences 5(5): 799-804. doi: 10.3923/ajps.2006.799.804

Wolf, F. R., Nonomura, A. M. and Bassham, J. A. (1985), GROWTH AND BRANCHED HYDROCARBON PRODUCTION IN A STRAIN OF BOTRYOCOCCUS BRAUNII (CHLOROPHYTA)1. Journal of Phycology, 21: 388-396. doi:10.1111/j.0022-3646.1985.00388.x

Optimization of Environmental Factors[edit]

Qin J.G., Lin Y., Optimization of the Growth Environment of Botryococcus braunii Strain CHN 357. Journal of Freshwater Ecology 21(1):169-176. doi:10.1080/02705060.2006.9664110

Rao A.R., Dayananda C., Sarada R., Shamala T.R., & Ravishankar G. A. (2007). Bioresource Technology. Effect of salinity on growth of green alga Botryococcus braunii and its constituents. Bioresource Technology, 98, (3): 560-564. doi: 10.1016/j.biortech.2006.02.007

Sakamoto K., Baba M., Suzuki I., Watanabe M.M., & Shiraiwa Y. (2012). Optimization of light for growth, photosynthesis, and hydrocarbon production by the colonial microalga Botryococcus braunii BOT-22. Bioresour Technol,110: 474-9. doi: 10.1016/j.biortech.2012.01.091.

Xu, L. , Wang, F. , Guo, C. and Liu, C. (2012), Improved algal oil production from Botryococcus braunii by feeding nitrate and phosphate in an airlift bioreactor. Eng. Life Sci., 12: 171-177. doi:10.1002/elsc.201100110

Yoshimura T., Okada S., & Honda M. (2013). Culture of the hydrocarbon producing microalga Botryococcus braunii strain Showa: optimal CO2, salinity, temperature, and irradiance conditions. Bioresour Technol, 133:232-9. doi: 10.1016/j.biortech.2013.01.095.

Nitrogen Focus[edit]

Singh, Y. & Kumar, H.D. World (1992). Lipid and hydrocarbon production by Botryococcus spp. under nitrogen limitation and anaerobiosis. J Microbiol Biotechnol, 8: 121. doi:10.1007/BF01195829

Seed Culture[edit]

Rao AR, Sarada, R., Gokare, R. & Phang S. (2014). Industrial Production of Microalgal Cell-Mass and Bioactive Constituents from Green Microalga-Botryococcus braunii. Recent Advances in Microalgal Technology. 1-19.

Shimamura R., Watanabe S., Sakakura Y., Shiho M., Kaya K. & Watanabe M. (2012). Development of Botryococcus Seed Culture System for Future Mass Culture. Procedia Environmental Sciences, 15: 80-89.


Lee K., & Kim T. (2002). Effects of harvesting method and growth stage on the flocculation of the green alga Botryococcus braunii. Letters in Applied Microbiology 27(1):14-18. doi:10.1046/j.1472-765X.1998.00375.x"